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Security of Testing Farm Service
Havlín, Jan ; Malinka, Kamil (referee) ; Drga, Jozef (advisor)
This thesis deals with security of Testing Farm Service in Red Hat company. Specifically, it is about unauthorized usage of testing machines for purposes which are not allowed. The need for implementing security measures comes from the fact that users are allowed to run arbitrary code on test machines as the user root. In the implementation part of the thesis, a monitoring agent was created and deployed to the testing machines of the production environment of the service. This system watches transmitted packets, system resources and configuration. Based on these observations, it creates metrics about the system behavior and sends them over to monitoring server Prometheus.
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Security of Testing Farm Service
Havlín, Jan ; Malinka, Kamil (referee) ; Drga, Jozef (advisor)
This thesis deals with security of Testing Farm Service in Red Hat company. Specifically, it is about unauthorized usage of testing machines for purposes which are not allowed. The need for implementing security measures comes from the fact that users are allowed to run arbitrary code on test machines as the user root. In the implementation part of the thesis, a monitoring agent was created and deployed to the testing machines of the production environment of the service. This system watches transmitted packets, system resources and configuration. Based on these observations, it creates metrics about the system behavior and sends them over to monitoring server Prometheus.
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Stability of protein complexes in the cytoskeleton of the eukaryotic flagellum
Pružincová, Martina ; Varga, Vladimír (advisor) ; Čajánek, Lukáš (referee)
The cilium/flagellum is a complex organelle protruding from the cell body and functioning in motility, sensing, and signalling. It is composed of hundreds of protein constituents, the majority of which comprise the flagellar cytoskeleton - the microtubule-based axoneme. Because the flagellum lacks ribosomes, its protein constituents have to be imported from the cell body and delivered to proper locations. Moreover, these proteins have to retain their function over a considerable length of time, despite the mechanical stress caused by flagellar beating and due to environmental exposure. This raises the question whether and where protein turnover occurs. Previously, it was established that Chlamydomonas reinhardtii flagella are dynamic structures (Marshall & Rosenbaum, 2001). In contrast, in the Trypanosoma brucei flagellum axonemal proteins are remarkably stable (Vincensini et al., 2018). However, the questions of axonemal assembly and stability were so far investigated only for a small number of proteins and during relatively short periods. Moreover, in these experiments expression of studied proteins was controlled by non-native regulatory elements. To elucidate the site of incorporation of proteins from all major axonemal complexes and to find out if and where the protein turnover occurs, T....
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Induction of left-right asymmetric gene expression by extracellular fluid flow in ventral node
Šimková, Kateřina ; Vegrichtová, Markéta (advisor) ; Mašek, Jan (referee)
Left-right asymmetry determines the orientation of visceral organs during gastrulation in the mouse embryo. The asymmetry originates in ventral node which is located on the anterior end of primitive streak. Cells of the ventral node possess motile nodal cilia. These cilia rotate and generate leftward flow of extra-embryonic fluid. The leftward flow initiates asymmetric expression of Nodal signalling pathways in nodal cells. Abnormal nodal flow and the interruption of asymmetric gene expression cause reversal arrangement of visceral organs, called situs inversus. The mechanosensing and chemosensing models are the main theories addressing the question of how the leftward nodal flow is sensed by nodal cilia. The mechanosensing model, also referred to as 'two cilia model', is based on two types of cilia, motile and immotile. Motile cilia generate nodal flow while immotile cilia act as mechanosensors with polycystin cation channels. According to the chemosensing model, morphogens are secreted and form gradient that induces asymmetric gene expression in the node. It is still unclear which model is the right one, but it is possible that the final model is combination of both.
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Sperm centrioles and their role in reproduction
Vlčková, Monika ; Frolíková, Michaela (advisor) ; Liška, František (referee)
Centrioles are evolutionarily conserved protein structures composed of microtubules. In somatic cells, centrioles serve as the basal body of cilia and flagella and allow the assembly of pericentriolar material, thereby creating the centrosome. Without centrosome, animal cells are not capable of nuclear division. Centrioles do not arise de novo and their formation always requires the presence of a preexisting centriole. Since there are no centrioles in the egg at the time of fertilization, unlike spermatozoa, sperm is the carrier of centrioles and therefore all of the centrioles of the emerging organism are of paternal origin. There are two centrioles in the sperm - a cylindrical shape proximal and a distal one, which is perpendicular to the proximal. The sperm centrioles are the basis for the formation of the sperm flagellum and after fertilization form the mitotic spindle of the zygote, necessary for equal DNA and cell distribution. It follows from the above that the presence of centrioles in sperm is essential in mammals and defects in their structure may lead to male sterility or embryo development disorders. However, sperm centrioles differ from somatic centrioles in their structure and behavior and understanding these differences is one of the important tasks of reproductive biology.
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Stability of protein complexes in the cytoskeleton of the eukaryotic flagellum
Pružincová, Martina ; Varga, Vladimír (advisor) ; Čajánek, Lukáš (referee)
The cilium/flagellum is a complex organelle protruding from the cell body and functioning in motility, sensing, and signalling. It is composed of hundreds of protein constituents, the majority of which comprise the flagellar cytoskeleton - the microtubule-based axoneme. Because the flagellum lacks ribosomes, its protein constituents have to be imported from the cell body and delivered to proper locations. Moreover, these proteins have to retain their function over a considerable length of time, despite the mechanical stress caused by flagellar beating and due to environmental exposure. This raises the question whether and where protein turnover occurs. Previously, it was established that Chlamydomonas reinhardtii flagella are dynamic structures (Marshall & Rosenbaum, 2001). In contrast, in the Trypanosoma brucei flagellum axonemal proteins are remarkably stable (Vincensini et al., 2018). However, the questions of axonemal assembly and stability were so far investigated only for a small number of proteins and during relatively short periods. Moreover, in these experiments expression of studied proteins was controlled by non-native regulatory elements. To elucidate the site of incorporation of proteins from all major axonemal complexes and to find out if and where the protein turnover occurs, T....
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Assessement of reasons of facade attack with external thermal contact insulation system (etics)
Zvěřina, Aleš
The fasade biotic attack with external thermal contact insulation system (ETICS) can occure due to many different reasons. There are often reasons which relate to humidity in building construction, it determinatives for fungus grow. But often is dismiss, that spare of fungus are placed at nearest surroundings of assesmented ETICS and with humidity dotation attack overlying surface of ETICS in risk places of building. The goal of article is to show procedures and methods including diagnostics methods of assessement of concrete building which was insulated in 2010.
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